Restriction Enzymes

The restriction enzyme data in DNADynamo is based on the REBASE database of restriction enzymes, which lists over 500 restriction enzymes with commercial suppliers. However, as many of these enzymes have identical target sites and cleavage properties, it is frequently unnecessary and more convenient to work with just a subset of the available enzymes. DNADynamo allows the generation of 'enzyme boxes' that contain a subset of the available enzymes. To select which enzyme box is used for a restriction enzyme analysis, use the 'Select Enzyme Box' button.

In general where ever you see an enzyme name you can double click on it using the mouse to open an enzyme information window that displays the recognition site, isoschizomers,enzymes with compatible sticky ends, and suppliers registered with the REBASE database.

DNADynamo displays tables of restriction sites, linear and circular graphic maps and detailed sequence/restriction maps using the enzymes in the currently selected enzyme box. The silent mutagenesis tool can help you introduce restriction sites without changing the corresponding protein sequence.