Tables of Restriction Sites

Pressing the 'All', 'Unique' or 'Absent' buttons on the buttons panel opens the Restriction Enzyme Site Table display, which is shown below together with a linked Graphic Map in the lower display area.


The table displays the number of times each enzyme in the currently selected enzyme box cuts within the DNA sequence. Changing the selected enzyme box updates the table accordingly.

The position of each site within the DNA sequence can be added to the display by pressing the 'Position' button.

The 'Filter' button can be used to limit the range of cut sites displayed. For example, selecting '<10' limits the display to showing enzymes that cut less than 10 times in the DNA Sequence. If the DNA Sequence is a construct created with the virtual subcloning tool you can press the 'Diagnostic' button to display only sites that cut at least once within the insert and once within the vector.

The 'Digest' button will display the theoretical size of DNA fragments generated by digestion with the selected enzymes. If the Sequence is a construct, a digest of the original vector (without insert) is also show.

Double clicking on enzymes within the table will bring up the enzyme information display.

In the example show above, a graphic restriction map has been displayed below the table by pressing the 'Map' button on the main buttons panel. Enzymes in the table may be selected by single clicking with the mouse. Selected enzymes are highlighted in blue and added to the graphic map below, which in the example is set to show only selected enzymes in the linked table.