DNADynamo can virtually subclone an 'Insert' into a 'Vector' from the Vectors Box. The Virtual Subcloning Tool is accessed by selecting 'Subclone into Vector' from the 'Vector' pull down menu. See here for a detailed explanation of how to do this. In addition to generating the spliced sequence a Vector Map is created where the 'insert' is placed in the existing map for the vector. With constructs you may use the 'Diagnostic' filter option in the restriction enzyme site table to show enzyme sites that cut at least once in the insert and once in the vector.