Loading Sequencing Data
Sequencing data is opened by selecting 'Import Sequencing File' or 'Import Multiple Sequencing Files' from the Sequencing menu or the 'Load More Data' button on the alignment editing buttons panel. DNADynamo can load most .abi and .scf files.

Note, OSX users will find the FileChooser for the 'Import Multiple Sequencing Files' option different from their usual FileChooser, which does not support the selection of MultipleFiles via JAVA at present. The structures of your filing system may appear somewhat different to what you are used to. Use the Ctrl and/or Shift keys along with the mouse to select multiple files.

In 'guide' mode, all sequences are aligned against the 'wildtype' guide sequence in the main sequence window. If no sequence is present in the main window, DNADynamo generates consensus sequences and aligns sequences as best it can. Manual editing and confirmation of all sequence data and alignments will be required.

To save the sequencing data in the DNADynamo file, press the 'Save' Button at the top of the Alignment display or select SaveAs from the File pull down menu. Press the 'Sequence' button to return to the main DNADynamo interface.

Placing the text-editing cursor on bases in a sequence will highlight the corresponding base call in the chromatogram data. Individual sequences may be removed by selecting the 'Remove Sequence' Button. Note that the sequence that is removed is the one that has the text-editing cursor and has its chromatogram displayed in the chromatogram display area.