To Create or Edit an 'Enzyme Box', select 'Edit Enzyme Boxes' from the Restriction Enzymes Menu. The Enzyme Box Editor Window is opened.
'Select All' selects all the available enzymes.
'Deselect All' deselects all the enzymes in the Box.
'Select Prototypes' Selects enzymes which REBASE defines as the prototype of a group of enzymes with identical specificity. If the prototype enzyme is not commercially
available, another enzyme in the group is selected.
'Select Non-Degenerates' selects enzymes which do not recognise degenerate sequences such as AccI (GTMKCC)
'Dynamo' selects enzymes which after cleavage generate either a blunt end or a cohesive end which is not degenerate.
'Select Blunts' selects enzymes which generate a blunt end following cleavage
'Select 5'Overhangs' selects enzymes which generate 5' overhangs following cleavage
'Select 3'Overhangs' selects enzymes which generate 3' overhangs following cleavage
'Select' on the 'length' panel selects recognition sites whose length lies equal to or between the selected values. Note that degenerate bases do not contribute to the length of the site in this selection model
The 'Company' panel allows selection of enzymes sold by a particular company as defined by REBASE.
Combining Group Selections
Pressing a 'group selection button' selects all the appropriate enzymes from the list of all available enzymes. However, sometimes it may be desirable to select a further subset of enzymes from those which are already selected. You can do this by pressing the 'SHIFT' key on the keyboard at the same time as selecting a group button For example, to select Prototypes that aren't degenerate, first press 'Select Prototypes' then press 'SHIFT' and 'Select Non-Degenerates'.