1) 'Select All' selects all the available enzymes.
2) 'Deselect All' deselects all the enzymes in the Box. This function is useful if you are starting to create a new Enzyme Box form scratch.
3) 'Select Prototypes' Selects enzymes which REBASE defines as the prototype of a group of enzymes with identical specificity. If the prototype enzyme is not commercially
available, another enzyme in the group is selected.
4) 'Select Non-Degenerates' selects enzymes which do not recognise degenerate sequences such as AccI (GTMKCC)
5)'Select Blunts' selects enzymes which generate a blunt end following cleavage
6) 'Select 5'Overhangs' selects enzymes which generate 5' overhangs following cleavage
7) 'Select 3'Overhangs' selects enzymes which generate 3' overhangs following cleavage
8) 'Select' X' base pair recognition sites selects enzymes whose recognition sequence is X base pairs long
9) The 'CompanyCombo' allows selection of enzymes sold by a particular company as defined by REBASE.
Press the 'SHIFT' key on the keyboard at the same time as selecting a group button with the mouse to select from the enzymes already selected. For example, to select Prototypes that aren't degenerate, first press 'Select Prototypes' then press 'SHIFT' and 'Select Non-Degenerates'.